Fluorescence Plate Reader Black Plate

Top reading instruments rely on measuring reflected light above the wells.

Fluorescence plate reader black plate.

Which 96 well plate is recommended for alpha synuclein fluorescence in vitro assay with thioflavin t. If you have a top read option i would recommend that combined with a black plate. Corning 96 well black polystyrene microplate flat bottom clear black polystyrene plate ideal for fluorescent assays matrix active group tc treated pkg of individually wrapped sterile lid. Find null cls3603 msds related peer reviewed papers technical documents similar products more at.

The use of white plates will result in higher raw signals because the light is reflected maximally by the white color of the plate. The use of black plates will result in lower raw signals because the black color of the plate can quench the light. These plate provide higher signal to background and increased sensitivity compared to white or black plates in luminescent assays with cross talk similar to black plates. Molecular probes 96 well microplates for fluorescence based assays are black walled clear bottom 96 well microplates that minimize well to well crosstalk and autofluorescence and so are optimized for fluorescence based applications.

For luminescent assays white plates reflect light and will maximize light output signal. These are usually detection modes with a high signal yield and the use of a black microplate helps reducing background auto fluorescence and well to well crosstalk providing better signal blank ratios. If microplate well size is interchangeable consider investing in a microplate reader that can accommodate a multitude of plate types such as a 6 to 384 well format. Modern fluorescence microplate readers offer typically the option to read the fluorescence signal both from the top and from the bottom.

The primary difference between white and black plates is their reflective properties. Because of this time resolved fluorescence assays can be run in either black or white plates. Instruments have become more compact to accommodate limited bench space without compromising reader intricacy or measurement speed. These plates are compatible with standard fluorescence microplate readers and are offered in a convenient 10.

For multiplexed luminescent and fluorescent assays using a white plate will support maximum light output signal for the. Consequently these plates are well suited for fluorescence intensity including fret and for fluorescence polarization assays. For fluorescent assays black plates absorb light and reduce background and crosstalk. In my experience this is best for fluorescence eliminating cross talk and reducing background fluorescence.

Alphaplate microplates are light gray plates designed to reduce cross talk while maintaining high signal in luminescent assays run in 384 well and 1536 well format. Thus white plates are commonly used for.